Table 8-1: Summary of all the blooms in the tank

Bloom Date Seawater
Source
(f=filtered)
Nutrients added (f /2) Algal Growth Lowest pCO2
(ppm)
Highest CO2 t.v. (cm hr-1) Distinct Enzyme effect? Surface film at end? Temperature and stirring range
(T / C, stir / rpm)
Other Parameters measured
(in addition to CO2 fluxes)
Dunaliella 1
(green alga)
December 1995 MAFF ship
(unfiltered)
N, P Intense 12 12.5 Yes Yes T 15 ± 1.5
stir 13.5 ± 1.5
SF6 / Ox tv.
Dunaliella 2 Jan / Feb 1996 Lowestoft laboratory supply (f)

N, P,

Weak 130 5.8 Yes No T 15 ± 2
stir 13.5 ± 2
SF6, Ox, tv, Alk, Chl,

effect of CA inhibitor

Phaeodactylum (diatom) Feb / March 1996 Lowestoft laboratory supply (f) N, P, Si Intense 7 19.5 Possibly at lowest pCO2 Yes T 15 ± 2
stir 13 ± 2
SF6 / Ox tv

Alk / TCO2 / pH (only at end) ,Chl (x2)

Amphidinium
(dinoflagellate)
April
1996
Lowestoft laboratory supply + boat off Yarmouth (f) N, P Intense 13 9.5 No No T 15.0 ± 1
stir 13.3 ± 0.5
(except 22 April)
Alk / TCO2 / pH (x6), Chl (x3)

effect of CA inhibitor

North Sea spring bloom (mostly Phaeocystis)

May / June 1996 Yarmouth boat
(in tank after <3 hrs -unfiltered)
none except 2nd week of 3rd sample (N,P) Low compared to cultures 160 (tank)
240 (sea)
4.6 Possibly No T 15.0 ± 1 (tank)
T 9 => 15 (sea)
stir 13.3 ± 0.5
Alk / pH / TCO2 (x8), Chl (x4)

SF6 tv.

Dunaliella 3 August
1996
Yarmouth boat
(f)
N, P Very weak 90 4.8 No No T 15.0 ± 0.8
stir 13.0 ± 0.4
Alk / TCO2 / pH (x2)
Dunaliella 4 Sept / Oct 1996 Yarmouth boat +30% artificial seawater (f) N, P
+trace metals & vitamins
Intense 8 13.0 Yes Yes T 15.0 ± 0.4
stir 13.0 ± 0.3
Alk / pH (x5), TCO2 (x11) Chl (x3)

SF6 tv.

Skeletonema
(Diatom)
August
1997
Lowestoft laboratory supply (f) N, P, Si
+trace metals & vitamins
Medium 64 4.6 No NoN No T 15.0 ± 0.4
stir 13.0 ± 0.3
Alk / TCO2/ pH (x2), Chl (x3)

Emiliana Huxleyi
(Coccolithophorid)
Sept /Oct 1997 Lowestoft laboratory supply (f) N, P (f / 20)
+trace metals
& vitamins
Medium 250 (Ehux bloom)
8 (film at end)
6 (Ehux bloom)
15 (film at end)
Yes Yes T 15.0 ± 0.4
stir 13.0 ± 0.3
Alk / pH / TCO2 (x5), Chl (x4)

More details about the sources of seawater, and the components of f /2 medium are given in sections 5.2.1 and 5.2.3.

Surface films are discussed in section 8.5. For justification of the "distinct enzyme effect" see section 8.4.